Class: Bio::Ensembl

Inherits:
Object show all
Defined in:
lib/bio/io/ensembl.rb,
lib/bio/io/ensembl.rb

Overview

Codes for backward-compatibility.

Defined Under Namespace

Classes: Base, Human, Mouse

Constant Summary collapse

ENSEMBL_URL = 
'http://www.ensembl.org'
EBIServerURI = 
ENSEMBL_URL

Instance Attribute Summary collapse

Class Method Summary collapse

Instance Method Summary collapse

Constructor Details

#initialize(organism, server = nil) ⇒ Ensembl

Returns a new instance of Ensembl.



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# File 'lib/bio/io/ensembl.rb', line 73

def initialize(organism, server = nil)
  @server = server || ENSEMBL_URL
  @organism = organism
  @uri = [ @server.chomp('/'), @organism ].join('/')
end

Instance Attribute Details

#organism ⇒ Object (readonly)

Organism name. (ex. ‘Homo_sapiens’).



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# File 'lib/bio/io/ensembl.rb', line 71

def organism
  @organism
end

#server ⇒ Object (readonly)

Server URL (ex. ‘www.ensembl.org’)



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# File 'lib/bio/io/ensembl.rb', line 68

def server
  @server
end

Class Method Details

.human ⇒ Object 



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# File 'lib/bio/io/ensembl.rb', line 79

def self.human
  self.new("Homo_sapiens")
end

.mouse ⇒ Object 



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# File 'lib/bio/io/ensembl.rb', line 83

def self.mouse
  self.new("Mus_musculus")
end

.server_uri(uri = nil) ⇒ Object 



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# File 'lib/bio/io/ensembl.rb', line 204

def self.server_uri(uri = nil)
  if uri
    @uri = uri
  else
    @uri || EBIServerURI
  end
end

Instance Method Details

#exportview(*args) ⇒ Object

Ensembl ExportView Client.

Retrieve genomic sequence/features from Ensembl ExportView in plain text. Ensembl ExportView exports genomic data (sequence and features) in several file formats including fasta, GFF and tab.

Examples

human = Bio::Ensembl.new('Homo_sapiens')
  or
human = Bio::Ensembl.human

# Genomic sequence in Fasta format
human.exportview(:seq_region_name => 1, 
                 :anchor1 => 1149206, :anchor2 => 1149229)
human.exportview(1, 1149206, 1149229)

# Feature in GFF
human.exportview(:seq_region_name => 1, 
                 :anchor1 => 1149206, :anchor2 => 1150000, 
                 :options => ['similarity', 'repeat', 
                              'genscan', 'variation', 'gene'])
human.exportview(1, 1149206, 1150000, ['variation', 'gene'])

Feature in TAB

human.exportview(:seq_region_name => 1, 
                 :anchor1 => 1149206, :anchor2 => 1150000, 
                 :options => ['similarity', 'repeat', 
                              'genscan', 'variation', 'gene'],
                 :format => 'tab')

Arguments

Bio::Ensembl#exportview method allow both orderd arguments and named arguments. (Note: mandatory arguments are marked by ‘*’).

Orderd Arguments

  1. seq_region_name - Chromosome number (*)

  2. anchor1 - From coordination (*)

  3. anchor2 - To coordination (*)

  4. options - Features to export (in :format => ‘gff’ or ‘tab’)

    ['similarity', 'repeat', 'genscan', 'variation', 
 'gene']

Named Arguments

  • :seq_region_name - Chromosome number (*)

  • :anchor1 - From coordination (*)

  • :anchor2 - To coordination (*)

  • :type1 - From coordination type [‘bp’, ]

  • :type2 - To coordination type [‘bp’, ]

  • :upstream - Bp upstream

  • :downstream - Bp downstream

  • :format - File format [‘fasta’, ‘gff’, ‘tab’]

  • :options - Features to export (for :format => ‘gff’ or ‘tab’)

    ['similarity', 'repeat', 'genscan', 'variation', 
 'gene']



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# File 'lib/bio/io/ensembl.rb', line 148

def exportview(*args)
  defaults = {
    :type1 => 'bp', 
    :type2 => 'bp', 
    :downstream => '', 
    :upstream => '', 
    :format => 'fasta',
    :options => [],
    :action => 'export', 
    :_format => 'Text', 
    :output => 'txt', 
    :submit => 'Continue >>'
  }

  if args.first.class == Hash
    options = args.first
    if options[:options] and options[:format] != 'fasta' and options[:format] != 'tab' 
      options.update({:format => 'gff'}) 
    end
  else
    options = {
      :seq_region_name => args[0], 
      :anchor1 => args[1], 
      :anchor2 => args[2],
    }

    case args[3]
    when Array
      options.update({:format => 'gff', :options => args[3]}) 
    when Hash
      options.update(args[3])
    end

    if args[4].class == Hash
      options.update(args[4])
    end
  end

  params = defaults.update(options)

  result, = Bio::Command.post_form("#{@uri}/exportview", params)

  return result.body
end