Class: Bio::DB::Fasta::Region

Inherits:
Object
  • Object
show all
Defined in:
lib/bio/db/fastadb.rb

Overview

Class to wrap a region of a chromosome

Constant Summary collapse

BASE_COUNT_ZERO = 
{:A => 0, :C => 0, :G => 0,  :T => 0}

Instance Attribute Summary collapse

Class Method Summary collapse

Instance Method Summary collapse

Constructor Details

#initialize(args = {}) ⇒ Region

Returns a new instance of Region.



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# File 'lib/bio/db/fastadb.rb', line 90

def initialize(args ={})
  @entry = args[:entry]
  @start = args[:start]
  @end = args[:end]
  @orientation = args[:orientation]
end

Instance Attribute Details

#allele_freq ⇒ Object Also known as: base_ratios

Returns the value of attribute allele_freq.



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# File 'lib/bio/db/fastadb.rb', line 88

def allele_freq
  @allele_freq
end

#average_coverage ⇒ Object

Returns the value of attribute average_coverage.



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# File 'lib/bio/db/fastadb.rb', line 88

def average_coverage
  @average_coverage
end

#bases ⇒ Object

Returns the value of attribute bases.



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# File 'lib/bio/db/fastadb.rb', line 88

def bases
  @bases
end

#called ⇒ Object

Returns the value of attribute called.



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# File 'lib/bio/db/fastadb.rb', line 88

def called
  @called
end

#consensus ⇒ Object

Returns the value of attribute consensus.



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# File 'lib/bio/db/fastadb.rb', line 88

def consensus
  @consensus
end

#coverages ⇒ Object

Returns the value of attribute coverages.



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# File 'lib/bio/db/fastadb.rb', line 88

def coverages
  @coverages
end

#end ⇒ Object

Returns the value of attribute end.



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# File 'lib/bio/db/fastadb.rb', line 86

def end
  @end
end

#entry ⇒ Object

Returns the value of attribute entry.



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# File 'lib/bio/db/fastadb.rb', line 86

def entry
  @entry
end

#orientation ⇒ Object

Returns the value of attribute orientation.



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# File 'lib/bio/db/fastadb.rb', line 86

def orientation
  @orientation
end

#pileup ⇒ Object

Returns the value of attribute pileup.



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# File 'lib/bio/db/fastadb.rb', line 88

def pileup
  @pileup
end

#reference ⇒ Object

Returns the value of attribute reference.



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# File 'lib/bio/db/fastadb.rb', line 88

def reference
  @reference
end

#snps ⇒ Object

Returns the value of attribute snps.



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# File 'lib/bio/db/fastadb.rb', line 88

def snps
  @snps
end

#start ⇒ Object

Returns the value of attribute start.



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# File 'lib/bio/db/fastadb.rb', line 86

def start
  @start
end

#total_cov ⇒ Object

Returns the value of attribute total_cov.



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# File 'lib/bio/db/fastadb.rb', line 88

def total_cov
  @total_cov
end

Class Method Details

.parse_region(reg_str) ⇒ Object

Returns a region object from a string in form “name:start-end”

Raises:



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# File 'lib/bio/db/fastadb.rb', line 156

def self.parse_region(reg_str)
  string = reg_str.delete("'")
  fields_1 = string.split(":")
  raise FastaDBException.new(), "Invalid region. #{string}" if fields_1.length != 2
  fields_2 = fields_1[1].split("-")
  raise FastaDBException.new(), "Invalid region. #{string}" if fields_2.length != 2

  reg = Region.new(:entry=> fields_1[0], :start=>fields_2[0].to_i, :end=>fields_2[1].to_i)

  if reg.end < reg.start 
    reg.orientation = :reverse
  else
    reg.orientation = :forward
  end
  reg
end

Instance Method Details

#allele_freq_for_base(base) ⇒ Object Also known as: base_ratios_for_base

TODO: Debug, as it hasnt been tested in the actual code.



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# File 'lib/bio/db/fastadb.rb', line 98

def allele_freq_for_base(base)
  @all_ratios = Hash.new unless @all_ratios
  unless @all_ratios[base]
    ratios = Array.new
    for i in (0..region.size-1)
      ratios << @allele_freq[i][base]
    end
    @all_ratios[base] = ratios
  end
  @all_ratios[base]
end

#calculate_stats_from_pile(opts = {}) ⇒ Object

Calculates the concensus, base ratios, coverages and total coverages in the region

  • min_cov minimum coverage to make a call (default 0)

  • min_per minimum representation to make make a call. If more than one base 

can be called, the IUAPC ambiguity code is returned



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# File 'lib/bio/db/fastadb.rb', line 117

def calculate_stats_from_pile(opts={})
  min_cov = opts[:min_cov] ? opts[:min_cov] : 0
  min_per =  opts[:min_per] ? opts[:min_per] : 0.20
  self.called = 0
  reference = self.reference.downcase

  self.allele_freq = Array.new(self.size, BASE_COUNT_ZERO) 
  self.bases = Array.new(self.size, BASE_COUNT_ZERO) 
  self.coverages = Array.new(self.size, 0)
  self.total_cov = 0

  self.pileup.each do | pile |

    if pile.coverage > min_cov
      self.allele_freq[pile.pos - self.start ] = pile.allele_freq
      reference[pile.pos - self.start   ] = pile.consensus_iuap(min_per).upcase
      self.coverages[pile.pos - self.start   ]  = pile.coverage.to_i
      self.bases[pile.pos - self.start       ]  = pile.bases
      self.called += 1 
    end
    #puts "#{pile.pos}\t#{bef}\t#{reference[pile.pos - region.start  - 1 ]} "
    self.total_cov += pile.coverage
  end

  self.consensus = Bio::Sequence.new(reference)
  self.consensus.na
  if self.orientation == :reverse
    self.consensus.reverse_complement!()
  end
  self.average_coverage = self.total_cov.to_f/self.size.to_f
  self
end

#size ⇒ Object Also known as: length

Length of the region



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# File 'lib/bio/db/fastadb.rb', line 174

def size
  @end - @start
end

#to_s ⇒ Object 



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# File 'lib/bio/db/fastadb.rb', line 150

def to_s
  string = @entry + ":" + @start.to_s + "-" + @end.to_s 
  string
end