Class: Bio::PAML::Codeml::PositiveSites

Inherits:
Array
  • Object
show all
Defined in:
lib/bio/appl/paml/codeml/report.rb

Overview

List for the positive selection sites. PAML returns:

Naive Empirical Bayes (NEB) analysis Positively selected sites (*: P>95%; **: P>99%) (amino acids refer to 1st sequence: PITG_23265T0)

        Pr(w>1)     post mean +- SE for w

17 I      0.988*        3.293
18 H      1.000**       17.975
23 F      0.991**       6.283

(…)

131 V      1.000**       22.797
132 R      1.000**       10.800

(newline)

these can be accessed using normal iterators. Also special methods are available for presenting this data

Instance Attribute Summary collapse

Instance Method Summary collapse

Constructor Details

#initialize(search, buf, num_codons) ⇒ PositiveSites

Returns a new instance of PositiveSites.

Raises:



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# File 'lib/bio/appl/paml/codeml/report.rb', line 537

def initialize search, buf, num_codons
  @num_codons = num_codons
  if buf.index(search)==nil
    raise ReportError,"No NB sites found for #{search}" 
  end
  # Set description of this class
  @descr = search
  lines = buf.split("\n")
  # find location of 'search'
  start = 0
  lines.each_with_index do | line, i |
    if line.index(search) != nil
      start = i
      break
    end
  end
  raise ReportError,"Out of bound error for <#{buf}>" if lines[start+6]==nil
  lines[start+6..-1].each do | line |
    break if line.strip == ""
    fields = line.split
    push PositiveSite.new(fields)
  end
  num = size()
  @buf = lines[start..start+num+7].join("\n")
end

Instance Attribute Details

#descr ⇒ Object (readonly)

Returns the value of attribute descr.



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# File 'lib/bio/appl/paml/codeml/report.rb', line 535

def descr
  @descr
end

Instance Method Details

#graph ⇒ Object

Generate a graph - which is a simple string pointing out the positions showing evidence of positive selection pressure.

>> c.sites.graph[0..32]
=> "                **    *       * *"



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# File 'lib/bio/appl/paml/codeml/report.rb', line 569

def graph
  graph_to_s(lambda { |site| "*" })
end

#graph_omega ⇒ Object

Generate a graph - which is a simple string pointing out the positions showing evidence of positive selection pressure, with dN/dS values (high values are an asterisk *)

>> c.sites.graph_omega[0..32]
=> "                24    3       3 2"



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# File 'lib/bio/appl/paml/codeml/report.rb', line 580

def graph_omega
  graph_to_s(lambda { |site| 
      symbol = "*"
      symbol = site.omega.to_i.to_s if site.omega.abs <= 10.0
      symbol
  })
end

#graph_seq ⇒ Object

Graph of amino acids of first sequence at locations



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# File 'lib/bio/appl/paml/codeml/report.rb', line 589

def graph_seq
  graph_to_s(lambda { |site |
    symbol = site.aaref
  })
end

#graph_to_s(func, fill = ' ') ⇒ Object

:nodoc: Creates a graph of sites, adjusting for gaps. This generator is also called from HtmlPositiveSites. The fill is used to fill out the gaps



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# File 'lib/bio/appl/paml/codeml/report.rb', line 604

def graph_to_s func, fill=' '
  ret = ""
  pos = 0
  each do | site |
    symbol = func.call(site)
    gapsize = site.position-pos-1
    ret += fill*gapsize + symbol
    pos = site.position
  end
  gapsize = @num_codons - pos - 1
  ret += fill*gapsize if gapsize > 0
  ret
end

#to_s ⇒ Object

Return the positive selection information as a String



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# File 'lib/bio/appl/paml/codeml/report.rb', line 596

def to_s
  @buf
end