Top Level Namespace

Includes:
Bio::MAF

Defined Under Namespace

Modules: Bio, BioMaf

Constant Summary collapse

INTERVAL = 
10
LOG = 
Bio::MAF::LOG
PRINTERS = 
{
  'flat' => :FlatPrinter,
  'stack' => :CallStackPrinter
}

Constants included from Bio::MAF

Bio::MAF::FASTA_LINE_LEN, Bio::MAF::TYPE_PROPS

Instance Method Summary collapse

Methods included from Bio::MAF

handle_logging_options

Instance Method Details

#apply_options(tiler) ⇒ Object 



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# File 'bin/maf_tile', line 30

def apply_options(tiler)
  tiler.reference = $options.ref if $options.ref
  tiler.species = $options.species
  tiler.species_map = $options.species_map
  tiler.fill_char = $options.fill_char if $options.fill_char
end

#build_index(maf, index) ⇒ Object 



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# File 'bin/maf_index', line 19

def build_index(maf, index)
  parser = Bio::MAF::Parser.new(maf, $options.parser_opts)
  idx = Bio::MAF::KyotoIndex.build(parser, index, $options.ref_only)
  idx.close
end

#desc(seq) ⇒ Object 



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# File 'bin/find_overlaps', line 7

def desc(seq)
  "#{seq.source}:#{seq.start}-#{seq.end}"
end

#each_tiler(access, intervals) ⇒ Object 



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# File 'bin/maf_tile', line 37

def each_tiler(access, intervals)
  intervals.each do |int|
    access.tile(int) do |tiler|
      apply_options(tiler)
      yield tiler
    end
  end
end

#handle_interval_spec(int) ⇒ Object 



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# File 'bin/maf_extract', line 25

def handle_interval_spec(int)
  if int =~ /(.+):(\d+)-(\d+)/
    if $options.one_based
      Bio::GenomicInterval.new($1, $2.to_i, $3.to_i)
    else
      Bio::GenomicInterval.zero_based($1, $2.to_i, $3.to_i)
    end
  else
    raise "Invalid interval specification: #{int}"
  end
end

#handle_list_spec(spec) ⇒ Object 



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# File 'bin/maf_extract', line 17

def handle_list_spec(spec)
  if spec =~ /^@(.+)/
    File.read($1).split
  else
    spec.split(',')
  end
end

#make_processing_task(maf) ⇒ Object 



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# File 'bin/maf_bgzip', line 59

def make_processing_task(maf)
  maf_base = File.basename(maf)
  base = maf_base.gsub(/\.maf.*/, '')
  bgz_path = "#{$options.dir}/#{base}.maf.bgz"
  if File.exist?(bgz_path) && ! $options.force
    LOG.error "#{bgz_path} already exists, refusing to overwrite " \
    "without --force!"
    exit 1
  end
  idx_path = nil
  if $options.index
    idx_path = "#{$options.dir}/#{base}.kct"
    if File.exist?(idx_path) && ! $options.force
      LOG.error "#{idx_path} already exists, refusing to overwrite " \
      "without --force!"
      exit 1
    end
  end
  lambda { process_maf(maf, bgz_path, idx_path) }
end

#parse_interval(line) ⇒ Object 



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# File 'bin/maf_tile', line 9

def parse_interval(line)
  src, r_start_s, r_end_s, _ = line.split(nil, 4)
  r_start = r_start_s.to_i
  r_end = r_end_s.to_i
  i_src = if $options.bed_species
            "#{$options.bed_species}.#{src}"
          else
            src
          end
  if $options.one_based
    Bio::GenomicInterval.new(i_src, r_start, r_end)
  else
    Bio::GenomicInterval.zero_based(i_src, r_start, r_end)
  end
end

#process_maf(maf_path, bgz_path, idx_path) ⇒ Object 



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# File 'bin/maf_bgzip', line 80

def process_maf(maf_path, bgz_path, idx_path)
  maf_base = File.basename(maf_path)
  LOG.debug { "Processing #{maf_base}." }
  p = Bio::MAF::Parser.new(maf_path,
                           :retain_text => true)
  if idx_path
    if File.exists?(idx_path)
      File.unlink(idx_path)
    end
    idx = Bio::MAF::KyotoIndex.new(idx_path)
    idx.prep(bgz_path, :bgzf, $options.ref_only)
    exec = Bio::MAF::Executor.create
  end
  start_t = Time.now
  last_t = start_t
  last_pos = 0
  n_blocks = 0
  maf_size = File.size(maf_path)
  File.open(bgz_path, 'wb') do |out_f|
    Bio::BGZF::Writer.new(out_f, $options.level) do |bgz_w|
      maf_w = Bio::MAF::Writer.new(bgz_w)
      maf_w.write_header(p.header)
      p.each_block do |block|
        bgz_w.write(block.orig_text)
        if idx
          block.offset = bgz_w.last_write_pos
          exec.submit do
            idx.index_blocks([block])
          end
        end
        n_blocks += 1
        if n_blocks % 100 == 0
          cur_t = Time.now
          delta_t = cur_t - last_t
          if delta_t > INTERVAL
            cur_pos = p.phys_f.tell
            LOG.debug {
              pos_mb = cur_pos.to_f / 1048576
              delta_bytes = cur_pos - last_pos
              rate = delta_bytes.to_f / delta_t
              mb_rate = rate / 1048576
              pct = cur_pos.to_f / maf_size * 100
              elapsed = cur_t - start_t
              sprintf("%s: processed %.1f MB (%.1f%%) in %ds, %.2f MB/s.",
                                maf_base,
                                pos_mb,
                                pct,
                                elapsed,
                      mb_rate)
            }
            last_t = cur_t
            last_pos = cur_pos
          end
        end
      end
    end
  end
  unc = p.f.tell if p.f != p.phys_f
  p.close
  if idx
    exec.shutdown
    idx.db.synchronize(true)
  end
  elapsed = Time.now - start_t
  mb = maf_size.to_f / 1048576
  mb_rate = mb / elapsed
  LOG.info { sprintf("Processed %s (%.1f MB) in %ds, %.2f MB/s",
                     maf_base,
                     mb,
                     elapsed,
                     mb_rate) }
  if unc
    LOG.info {
      unc_mb = unc / 1048576
      unc_rate = unc_mb / elapsed
      sprintf("  Uncompressed: %.1f MB, %.2f MB/s",
              unc_mb, unc_rate)
    }
  end
  LOG.info {
    raw_size = unc || maf_size
    avg_block_kb = raw_size.to_f / n_blocks / 1024
    sprintf("  %d alignment blocks, average size %.2f KB",
            n_blocks, avg_block_kb)
  }
  LOG.info {
    orig_size = unc ? unc : maf_size
    bgzf_size = File.size(bgz_path).to_f
    ratio = bgzf_size / orig_size
    sprintf("  Compressed with BGZF (level=%d) to %.1f MB (%.1fx)",
            $options.level,
            bgzf_size / 1048576,
            ratio)
  }
end

#target_for(base, interval, &blk) ⇒ Object 



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# File 'bin/maf_tile', line 25

def target_for(base, interval, &blk)
  path = "#{base}_#{interval.zero_start}-#{interval.zero_end}.fa"
  File.open(path, 'w', &blk)
end

#usage(msg) ⇒ Object 



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# File 'bin/maf_extract', line 127

def usage(msg)
  $stderr.puts msg
  $stderr.puts $op
  exit 2
end